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4In 2007, Barrangou et al. demonstrated for the first time the function of CRISPR/Cas9 as a defensive mechanism of bacteria against bacteriophage attacks by a technique called “plaquing” which builds upon the technique of “plating” bacteria on agar. Plaques of viruses on agar are areas without growth of bacteria due to viral attacks.

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Quadtrees are everywhere spatial data exists. Mapping services use quadtree-like tile pyramids to serve map tiles at different zoom levels (Bing's quadkey system, for example, addresses tiles as base-4 paths). Game engines use them for collision detection and visibility culling. Geographic information systems use spatial indexes to store and query spatial datasets. PostGIS uses GiST indexes (R-tree-style) for spatial queries on geometries, while PostgreSQL's core supports quadtree-like SP-GiST indexes for certain data types like points.