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This anomaly is indicative of the larger challenge of culturing various microbial species, referred to as microbial “unculturability.” This cannot be explained by the use of agar alone or by the substitution of an alternative gelling agent, but rather by the difficulties in consistently recreating on an agar plate the multi-variable environment in which microbes grow naturally. Given such challenges, the risk of shortages, and the vulnerabilities of the agar supply chain, why is it so difficult to find suitable alternatives?

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He appeared in handcuffs and wearing an olive-green sweat suit during his arraignment Thursday evening in Manhattan criminal court. He wasn’t asked to enter a plea, and was released, pending his next court date on April 9.

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